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Figure 5.
Figure 5. Molecular Surfaces of eIF1A(A) Three views of the
electrostatic surface of eIF1A produced with the program GRASP
([33]). Positive charge is colored blue and negative red
(±40 kT). Surfaces to the right are progressively rotated
90° counterclockwise around the vertical axis of the paper.
eIF1A is in a different orientation from the other figures with
the far right panel looking down the axis/hole of the β barrel
(approximately 90° clockwise rotation of Figure 4B around
horizontal axis of paper). Secondary structure elements are
labeled for orientation.(B) Surface of amino acids that have
backbone amide chemical shift changes upon binding RNA are
colored green (same residues as Figure 4B). Yellow surfaces are
amino acids that have undetectable amide resonances due to
intermediate conformational exchange in the unbound protein and
are not available as probes of RNA binding. The three surfaces
have the same orientation as in (A).
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