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Figure 4.
Figure 4. T4 gene 59 protein C-domain. (a) A 1.45 Å
resolution 2F[o] -F[c] electron density map (contoured at 1s) of
the loop H8-H9 region where Pro140 is the first residue (or the
"i position") of an extended b-hairpin. The quality of the map
ensures proper assignment of the a[L] conformation of Gln143
(the fourth residue of the b-hairpin, b i+3) with Val142 (b i+2)
directed towards a solvent-accessible hydrophobic surface. (b)
"Top" view of the C-domain trace shows side-chains of several
a[L] residues (Glu128 in loop H7-H8, Gln143 in H8-H9, Asn154 in
H9-H10) and a cluster of exposed hydrophobic residues (Phe123,
Val127, Val129, Phe148, Leu150, Ile155, Leu181, Val182, and
Ile188) form the "top" surface of the C-domain. (c) "Bottom"
view of the C-domain trace shows the side-chains of the a[L]
conformation residue Asn169 and solvent-exposed residues
(Phe132, Phe136, Phe167, Phe208 and Ile209) clustered in a
hydrophobic patch forming the "bottom" surface of the C-domain.
An acidic patch (Asp165, Asp172, Asp175, Glu176, and Asp179) is
adjacent to a large cluster of basic residues located within the
central groove (Arg5, Lys191, Arg194, and Lys195).
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