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Figure 3.
Figure 3. Stereo picture of the electron density of the
substrate complexes. The coordinates of the substrate molecules
benzoic acid and propionic acid were left out of the entire
refinement and all map calculations in order to reduce model
bias. The density of the (2F[obs]−F[calc]) map is contoured at
the 1.0 σ level and colored green, the density of the
(F[obs]–F[calc]) difference map is contoured at the 4.0 σ
level and colored blue. (a) Stereo picture of the electron
density for the complex of CPO-T with benzoate. Residual
electron density at Ser98 indicates the alternative, lower
occupancy, side-chain conformation. (b) Stereo picture of the
electron density for the complex of CPO-F with propionate.
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