|
Figure 3.
Recognition of 8-oxo-dGMP by MutT. A, hydrogen bonding
interactions between 8-oxo-dGMP and loop regions (apo in gray
and MutT-8-oxo-dGMP in slate). Amino acid residues involved in
the hydrogen bonding interactions are shown in ball and stick
representation. Water molecules are in red. Hydrogen bonds are
shown as yellow dashed lines. B, the hydrophobic cave composed
of β-1, β-3, β-3′, β-5, and α-2 is represented as a
translucent surface (carbon in white, nitrogen in cyan, and
oxygen in pink). C, interactions for the syn conformation of
8-oxo-dGMP. The hydrogen bond between Oδ of Asn-119 and N7-H of
8-oxoG is shown as a red dashed line. D, van der Waals
interactions around the O8 atom. Amino acid residues recognizing
O8 are shown in ball, stick, and translucent surface. E, a 2F[o]
− F[c] electron density map around 8-oxo-dGMP contoured at 1.5
σ (stereo view).
|
