|
Figure 3.
Crystal structures show canonical dimer interface and
destabilized loops. a, superposition of κI O18/O8 (blue) and
AL-09 H87Y (orange) shows restored dimer interface. b,
differences in the loop between Lys-39 and Pro-44 are visible in
the global superposition of κI O18/O8 (blue) and κI O18/O8
Y87H (red). c, superposition of κI O18/O8 (blue) and κI O18/O8
N34I/Y87H (green) shows a change in the same loop as observed in
b. d, shift of Pro-40 and Gly-41 in the κI O18/O8 Y87H mutant
(pink) disrupts an interaction between Tyr-87 and the carbonyl
of Gly-41. e, detailed view of Pro-40 and Gly-41 of κI O18/O8
(blue) and κI O18/O8 N34I/Y87H (green) shows same disrupted
interaction as observed with the κI O18/O8 Y87H mutant in d. f,
hydrogen bonding between the backbone carbonyl of Lys-42 and the
OH of Tyr-87 in κI O18/O8 (blue) is lacking with the His-87
mutation in κI O18/O8 Y87H (pink). This is also observed in the
κI O18/O8 N34I/Y87H mutant (not pictured).
|
