Figure 3.
Analysis of an alignment of the Jagged/Delta family DSL
domains representing various species (H. sapiens Jagged-1,
residues 187–229; D. melanogaster Serrate, residues 237–279;
H. sapiens Jagged-2, residues 198–240; H. sapiens Delta-like
1, residues 179–221; D. melanogaster Delta, residues
184–226; H. sapiens Delta-like 4, residues 175–217; C.
elegans LAG-2, residues 124–166; H. sapiens Delta-like 3,
residues 178–215) (a) and the DSL structure (b) reveals a
series of highly conserved, but surface-exposed, residues.
Residues that are conserved and predicted to form a Notch
binding face are shown in red, cysteines in yellow and a
nonconserved residue on the opposite face in blue. (c)
Electrostatic surface potential of J-1[DSL-EGF3] and
N-1[11–13] plotted at 4
kT e^-1 using APBS^54. Note the positively charged patch (blue)
within the DSL domain of Jagged-1 and the negatively charged
surface (red) of Notch. The surfaces predicted by
sequence/structure analysis (J-1[DSL-EGF3]) and NMR studies
(N-1[11–13]) to be involved in binding are highlighted by
green bands.
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
Nat Struct Biol
(2008,
15,
849-857)
copyright 2008.
4
kT e^-1 using APBS^54. Note the positively charged patch (blue)
within the DSL domain of Jagged-1 and the negatively charged
surface (red) of Notch. The surfaces predicted by
sequence/structure analysis (J-1[DSL-EGF3]) and NMR studies
(N-1[11–13]) to be involved in binding are highlighted by
green bands.