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Figure 3.
Contacts between NKG2A/CD94 and HLA-E/peptide. (A) Molecular
surface representations are shown of NKG2A (red), CD94 (green),
and HLA-E (blue), with the peptide shown in an all-atom
representation (N terminus on the left). The complex has been
splayed open to reveal the interface, with regions on each half
of the complex colored by the molecule making reciprocal
contacts. Coils have been included on the right to indicate the
position of the HLA-E α-helices from the complex relative to
NKG2A/CD94. (B) Contacts between NKG2A/CD94 (Left) or TCR KK50.4
(Right) and HLA-E are shown, with the molecules shown as
ribbons, colored as in A. The TCR α chain is purple, and the
TCR β chain is green. (C) The complex is shown as in A, but
with interface residues colored by a heat map (mutations with
>10-fold effect on affinity: red for HLA-E or salmon for CD94;
3- to 5-fold effect: yellow; 2-fold or less: blue) based on the
previous alanine mutagenesis analysis (12). (D) Schematic
representation of the binding footprints of NKG2A/CD94 (yellow)
and TCR KK50.4 (blue) on HLA-E.
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