Figure 3.
Fig. 3. Alterations in the NADP binding site changes the rate
of induction in vivo. GAL1 mRNA expression as a function of time
after galactose induction. Data are shown for wild-type Gal80p
and for Gal80p point mutants. All data were normalized to RNA
levels measured for a control gene, PMA1. A gal80 mutant has a high
expression level even when uninduced—as high as that seen for
wild-type Gal80p when fully induced. The dimer mutant, N230R,
also shows expression in the uninduced state (SOM text).
The above figure is reprinted
from an Open Access publication published by the AAAs:
Science
(2008,
319,
1090-1092)
copyright 2008.
mutant has a high
expression level even when uninduced—as high as that seen for
wild-type Gal80p when fully induced. The dimer mutant, N230R,
also shows expression in the uninduced state (SOM text).