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Figure 3.
Figure 3. A cartoon of the suggested catalytic pathway. In
the clockwise direction, 2-OH of Fru-6-P is deprotonated by the
γ-phosphate moiety of ATP and a nucleophilic attack occurs. An
intermediate pentavalent phosphorane is formed, and the negative
charges generated on the planary oxygen atoms are stabilized by
Lys47, Lys168, and Mg^2+, and maybe a H^+ extracted from 2-OH.
Redistribution of the phosphorane electrons breaks a diester
bond between the bridge oxygen and γ-phosphorus atoms. The
leaving group is stabilized by a H^+. Eventually, the leaving
group (ADP) is stabilized through a salt-bridge to Lys168 to
finish the reaction.
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