Figure 3.
Figure 3 Charge, conservation and interaction surfaces of Rrp40
N.
Molecular surface representations of Rrp40: left, the same view
as that depicted in Fig 2; right, rotated by 180° along a
vertical axis. (A) Molecular surfaces are coloured blue and red
according to positive and negative electrostatic potential,
respectively. (B) The degree of sequence conservation among
Rrp40 orthologues is mapped on the surface representation. Dark
or light green indicates residues that are fully or partially
conserved in Rrp40 orthologues, respectively (compare with Fig
1). (C) The conserved residues of the 3–
4
loop and the residues affected on addition of Mn^2+ are shown in
magenta and cyan, respectively. (D) Model of Rrp40 N
in the context of the exosome, obtained by replacing one of the
Rrp4 subunits in the structure of the Archaeoglobus fulgidus
(Af) exosome by Rrp40 N.
The conserved residues in the 3–
4
loop of the S1 domain and those affected by the addition
of Mn^2+ are shown in magenta and cyan, respectively. The
AfRrp41 and AfRrp42 subunits are shown in blue and green,
respectively, and the two AfRrp4 subunits are shown in orange.
Ribbon and surface representations were generated with PyMOL
(http://pymol.sourceforge.net).
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
EMBO Rep
(2007,
8,
63-69)
copyright 2007.
N.
Molecular surface representations of Rrp40: left, the same view
as that depicted in Fig 2; right, rotated by 180° along a
vertical axis. (A) Molecular surfaces are coloured blue and red
according to positive and negative electrostatic potential,
respectively. (B) The degree of sequence conservation among
Rrp40 orthologues is mapped on the surface representation. Dark
or light green indicates residues that are fully or partially
conserved in Rrp40 orthologues, respectively (compare with Fig
1). (C) The conserved residues of the 
3–