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Figure 3.
Figure 3. Chemical modification of mutant and wild-type 30S
subunits in the presence of Ksg. Sequencing lanes are labeled
U, G, C and A. (a) Ksg footprint in the 794 region of the 16S
rRNA upon chemical modification with DMS. (b) Ksg footprint in
the 926 region of the 16S rRNA upon chemical modification with
kethoxal. The A794G mutation renders the DMS reactivity of this
base undetectable, and the G926A mutation makes this residue
unreactive to chemical attack by kethoxal; thus, Ksg binding to
ribosomes carrying a mutation at one residue was detected as
protection of the other residue. Ksg was used at 0, 64 and 320
 g
ml^-1 (triangles denote increasing concentration). WT, wild-type
rRNA.
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