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Figure 3.
FIGURE 3. Proposed mechanism for the BphK-catalyzed
reductive dehalogenation of 3-Cl HOPDA. The first half-reaction
involves tautomerization of the HOPDA molecule followed by
nucleophilic substitution to form a mixed disulfide. The second
half-reaction is a disulfide exchange. Crystallographic data
suggest that His-106 stabilizes the negative charge on Cys-10.
Enz, enzyme.
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