|
Figure 3.
Figure 3: The novel drug translocation pathway for AcrB.
Figure 3 : The novel drug translocation pathway for
AcrB. Unfortunately we are unable to provide accessible
alternative text for this. If you require assistance to access
this image, or to obtain a text description, please contact
npg@nature.com-
Bound minocycline in the binding protomer is shown in a CPK
representation and coloured as in Fig. 1. a, Stereo-pair of the
inner cavities inside the periplasmic region of AcrB viewed from
the side parallel to the membrane plane as in Fig. 1a. The
chicken-wire representation coloured in magenta is the
solvent-accessible inner cavity of the AcrB molecule created
with the program VOIDOO^42 and MAMA^43 of the Uppsala Software
Factory (http://xray.bmc.uu.se/usf/) with slight modifications
(surface-noise removal, and so on). For clarity, the access
protomer is removed. b, Close-up stereo views of the vestibules
in three different transport states, viewed from slightly
diagonally below to the side of the molecule. Three protomers
are superposed using the least-squares superposition program
LSQKAB, from the CCP4 program suite^33. The flexible region
above TM8 and the PC2 subdomain are represented by solid colours
corresponding to the colours in Fig. 1, and the remaining
main-chain tracings are more transparent. The vestibule is
indicated as a dotted circle.
|
