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Figure 3.
Details of the stabilizing interactions of the loop at the C
terminus of the engineered helix in the mutant L20/R63A in the
presence of guanidinium (A), methylguanidinium (B), and
ethylguanidi-nium (C). The superimposed F[o]-F[c] difference
maps contoured at 3[sigma] (red) define the position of the
ligands. The resolution of the maps is 1.45 A, 1.7 A, and 1.8 A,
respectively. The methylated and ethylated ligands adopt
alternative conformations as shown. (D) Interactions made by
Arg63 in the lysozyme (Molecule B, PDB code 262L). Similar
interactions are made by Arg52 in wild-type lysozyme.
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