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Figure 3.
Figure 3 Cell-adhesion assays. Insect Sf9 cells expressing
transmembrane RPTP  EGFP-fusion
constructs were observed by fluorescence microscopy. All RPTP
fusion
constructs (except for Ex -TM-EGFP)
have JM-D1-EGFP in the intracellular region, and are expressed
at the plasma membrane (indicated by white arrows); whereas EGFP
alone (control) is expressed uniformly in the cytosol. The
bright intracellular signal indicates overexpression of the
constructs in the endoplasmic reticulum and the Golgi apparatus.
Note the localization of MIF2, MIF3, Ex and
its mutants (L1m, L2m) at the cell–cell contact regions (also
see Figure 5B and C). IF14t and F14t fusion constructs (not
shown) did not induce cell aggregation. The Ex -TM-EGFP
construct contains only the first 10 intracellular residues of
RPTP .
Scale bar: 250 m
for the epifluorescence images; 20 m
(for constructs that form cell aggregates) and 10 m
(others) for the confocal images.
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