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Figure 3.
Figure 3. Lipoic acid binding in the LplA of T.
acidophilum. (a) 2F[o] -F[c] map of electron density from a
crystal of the native LplA soaked with R,S-lipoic acid. Lipoic
acid can be seen forming interactions with Arg72 which itself
forms a hydrogen bond with the carbonyl oxygen of Gly77. This
interaction is shown by the broken red lines. The bond distances
to the carbonyl oxygen are 3.06 and 2.86 Å. (b) Amino acid
residues in the lipoic acid binding site that are highly
conserved in LplAs. The residue numbers are shown in black using
T. acidophilum LplA numbering. (c) Comparison of the proposed
lipoic acid-binding sites in the T. acidophilum and E. coli
lipoate protein ligases. The T. acidophilum protein and lipoic
acid are coloured green whereas the E. coli protein and lipoic
acid are coloured blue.
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