|
Figure 3.
Figure 3: Methyllysine binding by human CHD1. a, The
bound-peptide structure of H3K4me3 (green), H3K4me1 (yellow),
H3K4me3R2me2a (cyan) and H3K4me3T3ph (red). b, The aromatic
cages from HP1 (blue) and Polycomb (cyan) with methyllysines 9
and 27. c, Fluorescence polarization peptide binding assays
using human or yeast CHD1. Using H3K4me3 peptide, human CHD1
(black) binds with a K[d] of 5  M,
W67L-human CHD1 (cyan) does not bind, W64L-human CHD1 (yellow)
binds with a K[d] of 290 M,
and budding yeast CHD1 (red) does not bind. W64L and W67L-human
CHD1 do not allow binding to H3K4me1 or the unmodified peptide.
d, Effect of adjacent peptide modifications. Black, H3K4me3;
cyan, H3K4me3R2me2a; red, H3K4me3T3ph.
|
