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Figure 3.
A close-up view of a metal ion binding by the HUH motif. A,
2F[o] - F[c] electron density map around the Mn^2+ binding site.
Residues near the HUH motif (His^60 and His^62) are shown in
sticks. Octahedral coordination of Mn^2+ is represented by black
dotted lines, whereas blue dotted lines denote hydrogen bonds.
Red balls represent water molecules. B, superposition of the
Mn^2+-bound (orange sticks) and Mn^2+-free (cyan sticks) S.
solfataricus transposase structures. Black dotted lines indicate
Mn^2+ coordination, whereas green dotted lines denote hydrogen
bonds. C, superposition of the Mn^2+-bound S. solfataricus
transposase (orange sticks) and Mn^2+-free structure of the H.
pylori IS608 transposase (green sticks). Black dotted lines
denote Mn^2+ coordination and blue dotted lines, hydrogen bonds.
D, binding of a divalent metal ion by the HUH motif in other
proteins. Black dotted lines denote Mn^2+ coordination and
hydrogen bonds.
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