Figure 3 - full size

Figure 3. Interaction of M.HhaI with duplexes where AP is opposite or adjacent to the target base. (a) The sequences of the 10 bp at the centre of the APadj duplex (left) and APopp duplex (right). Bases in the M.HhaI recognition sequence are shown in boldface/italic; the target base is circled; AP is denoted P and is in red; M is 5-methyl cytosine (used to direct enzyme binding to the opposite strand of the duplex). (b) Crystal structures of the complexes of the M.HhaI enzyme with the APadj duplex (left) and the APopp duplex (right), showing the molecular structure in the vicinity of the recognition sequence. (c) Detailed view of the H-bond interactions between the M.HhaI enzyme and the APadj duplex (left) and the APopp duplex (right).

The above figure is reprinted from an Open Access publication published by Oxford University Press: Nucleic Acids Res (2005, 33, 6953-6960) copyright 2005.