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Figure 4.
Fig. 4. Superposition ofapo-azuin form 2 (monomer D, thick lines) on Cu(ll) azurin thin lines). Differences between the two structures are entred
at l-lis 4~ nd His j iv, The side chain of His = 7 is shlrtcd by 1.6 toward the protein urface without changig the orientation of the imidazole ring.
The surface water molecule bou,ad to His = ~7 Ne shifts accordingly. Movement of Met ~ and Phe t~a side chaiqs prvides the space necessary for the
His ~7 swinging motion, The His 4~ side chain is moved in the opposite direction (0,6 A). This shift results in a slight positionl adjustment (0.4 A)
f the adjacent main chain of residues 8 to 10.
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