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Figure 3.
Figure 3
Stereo diagrams depicting the aluminium-binding sites. Electron-density maps (2F[o] -
F[c], contoured at 1 [sigma] ; green) are shown for the Al3+-binding sites in (a) the
N-lobe and (b) the C-lobe. Electron-density omit maps (F[o] - F[c], contoured at 3
[sigma] ; purple and orange) were calculated after refinement using a model in which
Al3+ ions or bicarbonate anions were omitted. The final model is superimposed in a stick
presentation with atoms in standard colours for the Al3+-coordinating residues (Asp60,
Tyr92, Tyr191 and His250 for the N-lobe; Asp395, Tyr431, Tyr524 and His592 for the C-lobe)
and bicarbonate anions. Al3+ atoms are shown by small spheres coloured cyan. The figures
were prepared with the programs BobScript (Esnouf, 1999 [Esnouf, R. M. (1999). Acta
Cryst. D55, 938-940.]-[bluearr.gif] ) and Raster3D (Merritt & Bacon, 1997
[Merritt, E. A. & Bacon, D. J. (1997). Methods Enzymol. 277, 505-524.]-[bluearr.gif]
).
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