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Figure 3.
Figure 3: The Rho -mDia[N] interaction. a, Stereo diagram of
the Rho -mDia[N] interface. Colour-coding of mDia[N] as in Fig.
2a, Rho in yellow, and switch regions of Rho in red. Residues
involved in the interface (cutoff level 3.6 Å) are shown in
ball-and-stick configuration. b, Schematic drawing of
interacting residues in Rho and mDia. c, Superimposition of the
Rho -mDia[N] complex (yellow) with RhoA  GDP
(red)27 (Protein Data Bank entry 1DPF). d, Superimposition of
the Rho -mDia[N] complex with Cdc42 GppNHp
(ref. 30, Protein Data Bank entry 1AM4). e, Van der Waals
surface representation of mDia[N] (upper) and RhoC GppNHp
(lower). Left panels show residues that are 100% conserved
between RhoA, B, C, Cdc42 and Rac1 (black, lower panel), or >75%
(blue) or >50% (magenta) conserved between mDia1, 2, 3 and human
Dia1 -3 (upper panel). Residues of Rho and mDia involved in
forming the Rho -mDia interface are shown in red in right panels.
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