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Figure 3.
FIG. 3. Structure of T-p53C mutants H168R and R249S. A,
superposition of C  atoms in the structures
of T-p53C-H168R (magenta) and T-p53C-R249S (yellow) on the
structure of T-p53C (PDB code 1UOL [PDB]
, molecule A; black). C atoms before and after
chain breaks are marked with spheres in the color of the
respective chain. B, structure of T-p53-R249S (yellow)
superimposed on the structure of p53 wild type (PDB code 1TSR
[PDB]
, molecule A; light gray). The zinc ion in both structures is
shown as a gray or yellow sphere. * denotes wild type residues.
Cys^238 in the structure of T-p53C-R249S was refined in two
alternative conformations, both contacting the zinc ion. Only
the conformation that was refined with higher occupancy (0.7) is
shown. C, stereo view of the final (2F[o] – F[c]) electron
density map at 1.9 Å resolution for mutant T-p53C-R249S
showing the peptide segment Cys^242-Met^243, the zinc ion, and
two residues that make contact with Met^243. The orientation is
the same as in B. The contour level is at 1.2  .
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