|
Figure 3.
FIG. 3. Determinants of the ROCK specificity toward Rho. A,
stereoview of superimposed structures of RhoA (orange) (33),
Rac1 (yellow) (50), and Cdc42 (brown) (51), focusing on the
ROCK-binding region of RhoA. Amino acids are labeled according
to the standard numbering of RhoA. B, surface representation of
the GTPases in the same orientation as in A. The residues that
interact with ROCKI-RBD are colored as follows: blue, positively
charged nitrogen atoms; red, negatively charged oxygen atoms;
green, non-charged hydrophobic atoms; black, the oxygen atom of
Tyr-66. Phe-39, found to be crucial for the specificity toward
ROCKI, is shown in yellow.
|
