Figure 3.
FIG. 3. Stereoviews of the structural conformation of SBA
and the mutants complexed, respectively, with maltose. A, SBA
(pH 5.4); B, M51T (pH 5.4); C, E178Y (pH 5.4); D, E178Y (pH
7.1); and E, N340T (pH 5.4). The electron density map (green,
2F[o] - F[c], contoured at 1.0 ) and the residues
around the base catalyst and maltose are illustrated. The
hydrogen bonds are indicated by broken lines (black). The
mutated residues and the disordered conformations of maltose and
the side chain of Glu380 in M51T are cyan. The disrupted
hydrogen bonds in each mutants are indicated by broken lines
(pink). This figure was generated using BOBSCRIPT (56) and
Raster3D (55).
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2004,
279,
7287-7295)
copyright 2004.
) and the residues
around the base catalyst and maltose are illustrated. The
hydrogen bonds are indicated by broken lines (black). The
mutated residues and the disordered conformations of maltose and
the side chain of Glu380 in M51T are cyan. The disrupted
hydrogen bonds in each mutants are indicated by broken lines
(pink). This figure was generated using BOBSCRIPT (56) and
Raster3D (55).