Figure 3.
FIG. 3. Binding of ribose and ribose 5-phosphate to RbsD.
a, binding of D-ribose. The -pyranoside form of the
sugar is fitted into the final 2F[o] - F[c] map (1.95 Å,
1.1 ). The C-4-OH group
interacts with the C-terminal carboxyl group via a water
molecule. The density of this water molecule is weak or
unobserved in 2 of 5 binding sites. These weak interactions are
not shown. b, surface representation of the intersubunit cleft.
The bound ribose is displayed in CPK mode with the oxygen and
carbon atoms in red and black, respectively. The bound water
molecule is in blue. c, binding of D-ribose 5-phosphate. The
phosphorylated sugar is in the -furanose form, which
was determined at the beginning of the refinement. The final
2F[o] - F[c] map (2.05 Å, 1.1 ) is shown. His-20 from
one RbsD subunit is shown in cyan, and rest of the residues from
the adjacent subunit are shown in green. Figs. 1 and 3 were
prepared with the program Bobscript (26).
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2003,
278,
28173-28180)
copyright 2003.
-pyranoside form of the
sugar is fitted into the final 2F[o] - F[c] map (1.95 Å,
1.1 
). The C-4-OH group
interacts with the C-terminal carboxyl group via a water
molecule. The density of this water molecule is weak or
unobserved in 2 of 5 binding sites. These weak interactions are
not shown. b, surface representation of the intersubunit cleft.
The bound ribose is displayed in CPK mode with the oxygen and
carbon atoms in red and black, respectively. The bound water
molecule is in blue. c, binding of D-ribose 5-phosphate. The
phosphorylated sugar is in the