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Figure 3.
Figure 3. ALSCRIPT[34.] alignment of three phylogenetically
related groups of sequences, each containing Gln corresponding
to PhoE Gln22. The groups, whose relationship is clear from
phylogenetic analysis of a T-COFFEE alignment [35.] with
PROTDIST and PROTPARS of the PHYLIP package, [36.] are separated
by horizontal lines. For clarity, not all the sequences analyzed
are shown. The sequences determined in the Streptococcus
pyogenes MGAS315 genome[37.] represent the three determined S.
pyogenes genomes. Similarly, Streptococcus pneumoniae R6[38.]
and Listeria monocytogenes,[39.] were arbitrarily chosen as
respective representatives of the two streptococcal and two
listeria genomes sequenced. The Listeria monocytogenes sequence
with Genbank code 16802560 contains 18 further residues at the
N-terminal end, as indicated, which are not included in the
alignment. PhoE numbering and secondary structure (determined
with STRIDE[40.] for the high resolution phosphate complex) are
shown above the alignment. Boxes mark residues of the catalytic
core and shaded residues form the hydrophobic substrate-binding
site in PhoE. Highly conserved positions are emboldened and
totally conserved residues additionally italicized.
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