Figure 3.
Figure 3 (A) Ensemble of NMR structures of the LicT-CAT−RAT
complex showing the protein backbone (in red) with some of the
interacting amino acid side chains (in yellow), and the RNA
helix (phosphodiester backbone in purple and nucleotides in
standard atom colours). (B) MOLSCRIPT (Kraulis, 1991)
representation of the LicT-CAT dimer interacting with its RAT
hairpin target. The two CAT monomers, each composed of a
four-stranded antiparallel -sheet,
are coloured in red and blue. Some important side chains
interacting with the RNA are shown in ball-and-stick
representation. The RNA phosphodiester backbone is shown in
purple and the nucleotides are in standard atom colours. (C and
D) Stereo views showing the pseudo-symmetric recognition of the
RNA asymmetric internal loop 1 and loop 2, respectively, by each
CAT monomer. The nucleotides forming loop 1 (the A3−A27
sheared pair and the bulged-out A26) and loop 2 (the
U7−A9−G22 triplet and the bulged-out U8) are shown in
ball-and-sticks as well as the neighbouring canonical base pairs
(U4−A25 in loop 1, G6−C23 in loop 2). Relevant hydrogen
bonds between protein and RNA residues are indicated as dotted
lines.
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
EMBO J
(2002,
21,
1987-1997)
copyright 2002.
-sheet,
are coloured in red and blue. Some important side chains
interacting with the RNA are shown in ball-and-stick
representation. The RNA phosphodiester backbone is shown in
purple and the nucleotides are in standard atom colours. (C and
D) Stereo views showing the pseudo-symmetric recognition of the
RNA asymmetric internal loop 1 and loop 2, respectively, by each
CAT monomer. The nucleotides forming loop 1 (the A3−A27
sheared pair and the bulged-out A26) and loop 2 (the
U7−A9−G22 triplet and the bulged-out U8) are shown in
ball-and-sticks as well as the neighbouring canonical base pairs
(U4−A25 in loop 1, G6−C23 in loop 2). Relevant hydrogen
bonds between protein and RNA residues are indicated as dotted
lines.