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Figure 3.
Fig. 3. Comparison of different conformational states of
the bovine mitochondrial  - and  -subunits to
the chloroplast -complex
containing tentoxin. (A) Schematic representation of the peptide
backbone of three different conformations of the mitochondrial
- and -subunits
that represent different states of the catalytic cycle (12, 30).
Both subunits were superimposed on their C[  ]-atoms.
The [E], [DP], and
[TP] states
of the noncatalytic -subunit is
shown in gray, the closed conformation of the catalytic -subunit (
[DP] and
[TP]) is
drawn in red, and the open [E]state is
coloured in green. Positions of residues Arg-304 and
Glu-67,
equivalent to residues Arg-297 and
Asp-83 that
are arrested by the tentoxin molecule in the chloroplast
structure (B), are indicated by red and black arrows,
respectively. As indicated by the arrow on the right (A), a
shift form the closed to the open conformation of the catalytic
-subunit
which occurs during the catalytic cycle is associated with a
domain movement that involves the -interface
at Arg-304-
Glu-67.
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