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Figure 3.
Figure 3. (a) The bound trisaccharide molecule on the
active site of A1-III. The Figure shows the bound trisaccharide
molecule and the surrounding amino acid residues and water
molecules interacting with the trisaccharide. The trisaccharide
molecule is represented by means of an orange ball-and-stick
model. The side-chains of Tyr and Trp, Asn and Gln, Asp, Arg,
and His residues are colored yellow, green, red, cyan and
purple, respectively. The water molecules are shown as a filled
circle ( o ). The hydrogen bonds between trisaccharide and
protein residues or water molecules less than 3.25 Å are
shown as dotted lines. This Figure was drawn using the program
GRASP.[23] (b) The conformational change of A1-III induced by
the binding of trisaccharide products. A1-III and A1-III with
trisaccharide structures are represented as thin and thick
lines, respectively. The water molecules are shown as a filled
circle ( o ). The hydrogen bonds in trisaccharide, protein
residues or water molecules less than 3.25 Å are shown as
dotted lines. This Figure was prepared using the programs
TURBO-FRODO (Bio-Graphics) on a Silicon Graphics INDY computer
and Adobe Illustrator 5.5.
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