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Figure 3.
Figure 3. Energetics of the Interaction(A) Mapping of
structural features onto the TRADD-N sequence. The first line is
human TRADD and the second line is mouse TRADD with identical
residues shown as dashes. Solvent accessibility of each TRADD-N
residue when the TRAF2 structure is pulled away is shaded below
the sequences. Residues involved in TRAF2 interaction are
colored based on their total surface area burials and those
completely buried at the interface are labeled by asterisks.
TRADD residues involved in TRAF2 interaction are almost entirely
identical between the human and mouse sequences, suggesting a
functional conservation among the mammalian species.(B)
Characterization of the interactions of wild-type and mutant
TRADD-N with wild-type TRAF domain of TRAF2 using biosensor
analysis. The dissociation constants (K[D]) in μM and relative
to the wild-type interaction are shown, as well as the
calculated ΔΔG of the mutational effects (25°C).(C)
TRAF2-interacting surface of TRADD-N. Region I is shown in cold
colors (light blue, blue, dark blue, green, and purple) and
region II in different shades of red. Colors for each mutated
residue follow the same text color in (B).
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