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Figure 3.
Fig. 3. Stereoviews of AspAT bound to substrate analogs.
Only Arg292* and the substrate analogs are indicated for
clarity. a, the substrate analog is C4-PLP (the moiety of
coenzyme is labeled as PLP and that of substrate as C4). The
active-site conformation of the C4-PLP complex is almost
identical to that of substrate-free AspAT (8, 9). b, the
substrate analog is C5-PLP. Each substrate analog and the side
chain of Arg292* are superimposed onto the (F[o]  F[c]) omit
map contoured at 2.5  (a) and 3
(b) with
these atoms omitted. c, the structure of the C3-PLP complex
(magenta), C4-PLP complex (yellow), C5-PLP complex (cyan), and
C6-PLP complex (green). Note that the side chain orientation of
Arg292* changes drastically depending on substrate. These
figures were produced using the program O (23).
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