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Figure 3.
Figure 3. T4 gene 59 protein N-domain. (a) The 1.45 Å
resolution 2F[o] -F[c] electron density map (contoured at 1s) in
the N-domain region showing Trp41 in loop H1-H2 packed against
Pro7 in loop S1-H1. (b) "Top" view trace of the N-domain with
side-chains of a basic cluster (Lys52, Arg53, Arg54, Lys56,
Lys61, Lys65, Lys67, and Lys69) and an acidic cluster (Asp55,
Asp84, Asp89, Asp92, and Asp94). Asp94, an a[L] conformer, is
surrounded by exposed hydrophobic residues (Leu96, Val97 and
Phe98). Asp84 is adjacent to Ile87 and Trp86 at the domain
interface. A polar residue, Glu159, buried in the interface is
hydrogen bonded to the phenol oxygen atoms of Tyr102 and Tyr193.
(c) "Bottom" view of the N-domain trace with side-chains of
Asn40 and Trp41 near exposed hydrophobic regions (residues Leu4,
Met6, Pro7, Ile14, Leu22, Met25, Leu68, Leu73, Ile103, Leu106,
and Ile109) . The side-chain of Phe111, located on the first
turn of helix H7 is pointing directly into the solvent space,
unaffected by crystal lattice contacts. Ile109 packs against
Ile2 of strand S1 anchoring the N-terminal b-strand.
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