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Figure 3.
Figure 3 Structural features involved in non-sequence-specific
DNA recognition. (A) View of HMG-D protein from this structure
(cyan) superimposed on the structure of the LEF-1 -DNA complex
(Love et al., 1995) (PDB accession No. 2lef; coral) in the same
orientation as Figure 1C. Side chains, selected on the basis of
their potential involvement in DNA specificity, are shown.
Detailed view of the interaction of residue 10 from both HMG-D
(B) and LEF-1 (C). HMG-D protein is in cyan, LEF-1 protein is in
coral, DNA is in gray, and black dashed lines depict proposed
hydrogen bonds with distances between donors and acceptors
shown. The Ser10 hydroxyl oxygen of HMG-D makes water-mediated
interactions with adenine 6 N3 and thymine 7 O4'. The LEF-1
Asn10 makes direct hydrogen bonds to guanine 9 N3 and thymine 8
O2 (in this LEF-1 model).
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