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Figure 2.
Figure 2 Primary- and secondary-structure alignment. Sequence
alignment, obtained using the GCG program (adjusted manually) of
five RCC1 homologues (from top to bottom: human, hamster,
Drosophila, Schizosaccharomyces pombe and Saccharomyces
cerevisiae) and the protein product, RPGR/RP3 of the X-linked
retinitis pigmentosa gene, along with the secondary structure of
human RCC1. The seven blades are coloured as in Fig. 1. Residues
highly conserved in seven RCC1 homologues (including Xenopus and
Caenorhabditis albicans RCC1) are boxed; invariant residues are
also shown in bold. The structurally conserved residues among
different repeats of RCC1 and RPGR/RP3 are shaded brown. Alanine
mutations that perturb the GEF activity14 are shown in blue
(human); the mutation responsible for the tsBN2 (refs 1, 2)
phenotype is shown in pink (hamster); and RCC1 mutations in S.
cerevisiae and S. pombe^3-7,13 are shown in red. Asterisks
indicate truncated sequences.
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