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Figure 4.
Figure 4. Stereo Diagram Showing Electron Density at the
AdoMet-Binding Site and the Interactions between AdoMet and
Nearby Residues of AS11The F[o]-F[c] electron density map,
contoured at 2.5 σ, was calculated at 1.85 Å from a model
that did not contain the AdoMet molecule. As shown, the AdoMet
molecule is clearly defined including its donor methyl group.
Residues appropriately positioned to make either hydrogen
bonding or van der Waals contact with AdoMet are shown. Carbon
atoms/bonds are colored gray/white; sulfur, yellow; nitrogen,
blue; and oxygen, red. The 13 potential hydrogen bonds whose
lengths were in the range 2.5–3.3 Å are represented by
dashed green lines. For clarity, G68 (whose backbone carbonyl
oxygen forms the near vertical hydrogen bond with the
α-ammonium group of the AdoMet methionine moiety) is not
labeled. One of the three potential hydrogen bonds to the 3′
OH of the AdoMet ribose is almost completely hidden in the view
shown.
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