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Figure 2.
Figure 2: Rational tuning of the reduction potential of Az.
a, Plot of E^o at pH 7.0 versus log P for the Az mutants from
this study (here P is the partition coefficient of the side
chain of the residue between octanol and water; that is, it is a
measure of hydrophobicity). The lowest reported E^o (dashed
orange line) at pH 7.0 for any T1 cupredoxin before this study,
M86Q pseudoazurin, is indicated. The highest E^o (dashed purple
line) at pH 6.2 of any T1 cupredoxin variant before this study,
M148L Rc, is also indicated; its potential was not measured at
pH 7.0 owing to protein instability. Considering the pH trend of
the E^o of all cupredoxins, this reported value for M148L Rc
would be lower at pH 7.0. b, Plot showing the E^o value for each
azurin variant at pH 7.0 unless otherwise noted. Not only is the
redox potential of Az tunable to the extremes of the redox
potentials attainable by T1 cupredoxins, but to nearly any redox
potential within the range. Error bars,  1
s.d.
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