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Figure 2.
Fig. 2. Binding of the H^+-ATPase phosphopeptide QSYpTV and
cotylenin A to the amphipathic groove of 14-3-3. (a) Cotylenin A
(yellow, sticks and semi-transparent surface) and the H^+-ATPase
phosphopeptide QSYpTV (light blue, sticks and semi-transparent
surface) are occupying the entire length of the amphipathic
groove of 14-3-3 (sand surface), the extreme C-terminal Val956
of the peptide displays a hydrophobic contact to the tricarbon
cycle of the cotylenin molecule. (b) Stereo view of the electron
density for cotylenin A bound to 14-3-3 and the C-terminal tail
of the H^+-ATPase peptide (QSYpTV-COOH). The peptide and
cotylenin A are drawn in ball-and-stick mode, 14-3-3 is
displayed as a ribbon (color coding as in a). An omit electron
density map of cotylenin A (contoured at 3 σ) is shown in
black. (c) Stereo view of a structural overlay of the position
of the diterpene molecules and the H^+-ATPase phosphopeptide in
the 14-3-3 complex structure with fusicoccin A (blue and
magenta, respectively, PDB code 1O9F) and with cotylenin A
(yellow and cyan, respectively).
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