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Figure 2.
FIGURE 2. In vitro fibril formation indicated shorter lag
time for AL-09. a, ThT fluorescence measured at 1, 24, and 120 h
indicated that AL-09 (  ) formed fibrils within
24 h, whereas  I O18/O8 (  ) did not
(error bars were ± S.D. for n = 5; ^*, p value 0.05).
Even after 120 h, I O18/O8 had not formed
fibrils (^**, p value 0.0079). Complete amyloid formation
kinetics followed by ThT fluorescence is included in
supplemental Fig. S2 online. b, electron micrograph of AL-09 at
24 h (scale bar, 500 nm), confirming fibril formation. c, I O18/O8
fibril formation at 215 h (scale bar 100 nm) confirms the
earliest time point at which ThT fluorescence enhancement
occurred (supplemental Fig. S2 online).
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