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Figure 2.
Fig. 2. Comparison of the coordinates of MMP-12 X-ray and NMR
structures having the E219A mutation (1JK3 and 2POJ,
respectively), with X-ray structures lacking this inactivating
point mutation. Black squares indicate the average Cα RMSD of
the crystal structure of MMP-12(E219A) with batimastat bound
(1JK3)^31 to crystal structures with wild-type (1JIZ^32 and
1ROS^17) or F171D mutant sequence (1RMZ,^35 2OXU,^40 and
2HU6^48). The inhibitor-free solution structure with the E219A
mutation (2POJ) is compared to crystal structures of active
MMP-12 with F171D mutation (blue triangles; 2OXU),
NNGH-inhibited F171D variant (red triangles; 1RMZ), and
batimastat-inhibited E219A variant (green squares; 1JK3).
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