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Figure 2.
Figure 2. Proposed reaction mechanism of SPT. (a)
Transaldimination of internal aldimine (holo-SPT), I with
L-serine to form external aldimine, II; (b) deprotonation of
C^α by base to form quinonoid, III; (c) Claisen condensation to
form putative β-ketoacid-aldimine complex, IV; (d)
decarboxylation to form product quinonoid, V; (e) reprotonation
of quinonoid to form product external aldimine, VI (f) release
of product 3-ketodihydrosphingosine (KDS) and regeneration of I.
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