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Figure 2.
Figure 2: Interaction between the H[CC] domain of BoNT/B and the
recognition domain of Syt-II. a, Binding interface on BoNT/B
(circled in dashed line) reveals hydrophobic groove (green,
hydrophobic surface). b, Close-up view of the binding interface
of Syt-II (magenta) and BoNT/B (light blue) by rotating 90°
around the x axis. c, Truncation mutants of Syt-II were tested
for BoNT/B binding activity. d, Point mutants of Syt-II were
tested for BoNT/B binding activity. e, f, Various synaptotagmin
mutants, in which residues within the recognition domain were
interconverted between Syt-II and Syt-I, were tested for BoNT/B
binding activity. WT, wild type; GST, glutathione S-transferase.
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