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Figure 2.
Figure 2. Determinants of resistance to Ksg in the DASL. (a)
Location of the DASL relative to Ksg. DASL residues A1518 and
A1519 are shown (gold). Also shown are A792 and G1497 (gray),
Ksg (cyan) and distances from the DASL to these residues (in
Angstroms). (b) Post-transcriptional modification of the DASL.
Plasmid-encoded 16S rRNAs carrying mutations in the DASL (or
wild-type (WT) rRNAs) were isolated from ksgA^+ (lanes 3, 7, 11,
15, 19 and 23) or ksgA^- strains (lanes 4, 8, 12, 16, 20 and
24). The presence of the methyl groups at A1518 and/or A1519 in
16S rRNA is detected as a strong reverse-transcriptase stop in
primer extension^19. Sequencing lanes, containing rRNA isolated
from the ksgA^- strain, are included for every mutant.
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