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Figure 2.
Figure 2: Interactions of platensimycin with ecFabF(C163Q) and
comparison with the apo structure. a, Superposition of
platensimycin (yellow, thicker sticks) on ecFabF, with
thiolactomycin (green) and cerulenin (cyan) shown for reference.
Side chains discussed in the text are labelled and coloured as
described above. The side chains from apo ecFabF are coloured
magenta. b, Interactions between the benzoic acid ring of
platensimycin (yellow) and ecFabF(C163Q). Side chains from the
protein discussed in the text are labelled and coloured green.
c, Interactions of ecFabF with the amide linker and ketolide of
platensimycin. The colour scheme is the same as in b. d, The
solvent-accessible surface area of FabF, coloured according to
electrostatic potential. Platensimycin is depicted as a stick
figure and coloured yellow, and is shown to be partly exposed to
solvent. Platensimycin buries 345 Å^2 of
solvent-accessible surface area on ecFabF, as calculated with
areaimol^24,25. Of that surface area, 122 Å^2 is a direct
result of the ketolide portion of the molecule, highlighting its
important contribution to platensimycin binding. Significant
interatomic distances (in ångströms) are marked in b
and c with red dashed lines and numbers.
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