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Figure 2.
Fig. 2. The monosaccharide binding site of PAL. (A) Stereo
view of the monosaccharide binding site of PAL in absence of
bound carbohydrate. The electron density for four water
molecules that occupy the binding site is shown, together with
the hydrogen bond network these waters make with the protein.
Superimposed in black are the equivalent residues of the mannose
complex (subunit B in the asymmetric unit). The water molecules
clearly mimic the oxygen atoms of bound mannose (black, thick
lines). (B) Stereo view of the interactions of mannose in the
monosaccharide binding site. The beta-anomeric oxygen position
of mannose is drawn in light green, as is the corresponding
orientation of the side chain of Glu221. The second conformation
of the side chain of Glu221, which also adopts two conformations
but not correlated with the anomeric form of the bound mannose
is shown in dark green.
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