|
Figure 2.
Figure 2. Comparison of the Switch Regions of RasQ61G with
WT-Ras and RasA59G
(A) Superposition of the switch 1 (Sw 1)
and switch 2 (Sw 2) regions of the GppNp bound forms of WT-Ras
(yellow) (Pai et al., 1990) and RasQ61G (blue). The GppNp and
Mg^2+ ions are in ball-and-stick representation in purple and
green, respectively. The water molecule in WT-Ras responsible
for the nucleophilic attack on the γ-phosphate (W175) is shown
as a yellow sphere; the closest water molecule in the RasQ61G
structure is shown as a blue sphere.
(B) Superposition of
the switch regions of the GppNp bound forms of RasA59G (gold)
(Hall et al., 2002) and RasQ61G (blue). Dotted lines represent
hydrogen bonds. For simplicity, only a few residues in each
region are shown.
This figure was prepared with Molscript
(Kraulis, 1991) and Pymol (http://pymol.sourceforge.net/).
|
