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Figure 2.
Diagrams of the DUF16 domain of MPN010. (A) A stereo view of
a refined electron-density map around a phenyalanine-rich region
countered at 1.5[sigma]. The 2Fo-Fc map from finally refined
phases was calculated using all reflection data between 20 A and
1.8 A. The figure was generated using the program RIBBONS
(Carson 1991). The residues around the phenylalanine-rich region
are represented by ball-and-stick models (blue, nitrogen atoms;
red, oxygen; green, carbon). The front phenylalanines are Phe75
and the ones below are Phe72. (B) A stereo drawing of a C[alpha]
atom trace of the DUF16 domain of MPN010. Each model was colored
differently. Every 20th residue is numbered and represented by a
dot. The phenylalanine (blue) and glutamine (pink) residues that
occupy trimer interfaces are represented by a ball-and-stick
model. The figure was generated by MOLSCRIPT (Kraulis 1991). (C)
The electrostatic surface potential of the DUF16 domain of
MPN010. A molecular surface is created by the program GRASP
(red, negative; blue, positive; white, uncharged) (Nicholls et
al. 1991). (D) Local coiled-coil parameters plotted against the
residue numbers. The gray squares represent a coiled-coil radius
and the black diamonds indicate coiled-coil pitch. "SH" and "LH"
represent the range of the short and the long helical bundles,
respectively. The small arrows indicate the positions showing
properties of stutters.
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