|
Figure 2.
Fig. 2. Schematic representation of MutM-DNA complexes. (A) The
MutM LRC used as the basis for the design of the cross-linking
system. (B to D) Interrogation complexes showing the positioning
of MutM over the DNA duplex, with the target base pair in aqua.
The side chain of the helix-probe residue Phe^114 is indicated.
The numbering system for the base pairs and backbone phosphates
is as indicated. The curved green line denotes the thiol-bearing
tether engaged in a cross-link to Cys166. Each blue box
indicates the site of tether attachment to DNA, the position of
the target base pair, and the separation between them, here
referred to as the register. Dashed blue lines indicate the lack
of order in the oxoG recognition loop. (E and F) Overall view of
complexes CC1 (E) and IC1 (F). CC1 is a lesion recognition
complex (LRC) formed by disulfide cross-linking between MutM and
oxoG-containing DNA. IC1 is the corresponding interrogation
complex having MutM cross-linked to non-lesion-containing DNA.
Blue box denotes the target base pair, which is disrupted in (E)
and intact but buckled in (F).
|
