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Figure 2.
Figure 2. (A) Ribbon diagram of PH0574, which consists of a
large (green) domain and a small (orange) domain. The Zn^2+ ion
is located between the two domains. (B) Close-up view of the
Zn^2+ ion binding site. A strong peak in the anomalous
difference Fourier map was observed only at the zinc position.
Many water molecules were observed around the Zn^2+ ion,
although none of these are involved in coordination. The
anomalous difference Fourier map contoured at 20  (orange)
and 2F[o] - F[c] map contoured at 2 (blue)
are shown. (C) Zinc ion coordinate diagram. The coordination of
the Zn^2+ ion is mediated by the conserved histidine and
cysteine residues both in PH0574 (green) and in saThrRS (blue,
parentheses, PDB ID: 1NYR). (D) Structure of the putative active
site drawn with a van der Waals surface with charge. The cavity
accommodates an acyl serine.
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