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Figure 2.
Substrate binding to FGE. (a) The surface representation of
FGE shows a groove with the redox-active cysteine pair
Cys-336/Cys-341 (red surface) at one end. Pro-182 (green
surface) marks the site of a cross-link with a photoreactive
substrate peptide (7) and hence is also close to the substrate
binding site (8). (b) FGE–peptide complex. The peptide LCTPSRA
binds to Cys-341 via an intermolecular disulfide bond. The FGE
surface is colored according to electrostatic potential
(±10 kT), showing a negative patch close to the C
terminus of the peptide, which is neutralized by Arg-P73. (c)
Close-up of b rotated 45° clockwise showing the exquisite
surface complementarity of the peptide with FGE.
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